Analysis of MCP-1 Levels and TNF-alfa /Il-10 Ratio in First Degree  Relatives (FDR) Type 2 Diabetes Mellitus Subjects with High Fat Diet Treatment

Dante Saksono Harbuwono; Heri  Wibowo; Risty Yasmin Bonita

doi:10.36590/jika.v5i2.548

Authors

Dante Saksono Harbuwono Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran, Universitas Indonesia, Depok, Indonesia
Heri Wibowo Departemen Parasitologi, Universitas Indonesia, Depok, Indonesia
Risty Yasmin Bonita Program Studi Imunologi Ilmu Biomedik, Universitas Indonesia, Depok, Indonesia

DOI:

https://doi.org/10.36590/jika.v5i2.548

Keywords:

MCP-1, monocyte, TNF-alfa/IL-10, T2DM FDR

Abstract

Type 2 diabetes mellitus is a chronic metabolic disease, which involves a variety of pathogenic processes. Overall these processes result in loss of mass and/or function of pancreatic-cells which is manifested as hyperglycemia. The influence of familial factors appears to be involved in the initiation and development of T2DM through both genetic and non-genetic factors. In the same family environment as patients with diabetes, first-degree relatives of patients with diabetes show a 30-70% increased risk of developing diabetes. Insulin resistance and cell dysfunction have been identified in individuals with a family history of diabetes, even before the onset of T2DM symptoms. Monocyte chemoattractant protein-1 (MCP-1) is a CC-chemokine with an attractant effect on monocytes, memory T cells and basophils. Expression of adipose tissue MCP-1 and circulating levels were positively correlated with adiposity. Larger adipocytes are associated with insulin resistance. This study aims to determine the changes in MCP-1 expression on monocytes before and after treatment with a high-fat diet for five days. The results showed that there was a change in inflammatory activity which was indicated by a high ratio of TNF-alfa/IL – 10 in the FDR group so that it could increase MCP-1 activity. However, there was no relationship between serum MCP-1 levels and the percentage of CD14+CD16+ monocytes in both groups.

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Author Biographies

Dante Saksono Harbuwono, Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran, Universitas Indonesia, Depok, Indonesia

Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran, Universitas Indonesia, Depok, Indonesia

https://scholar.google.co.id

Heri Wibowo, Departemen Parasitologi, Universitas Indonesia, Depok, Indonesia

Departemen Parasitologi, Universitas Indonesia, Depok, Indonesia

https://scholar.google.co.id

Risty Yasmin Bonita, Program Studi Imunologi Ilmu Biomedik, Universitas Indonesia, Depok, Indonesia

Program Studi Imunologi Ilmu Biomedik, Universitas Indonesia, Depok, Indonesia

References

ADA [American Diabetes Association]. 2023. 2.Classi?cation and diagnosis of diabetes: Standars of Medical Care in Diabetes-2023. Diabetes Care, 46(Supp 1) : S19-S40. https://doi.org/10.2337/dc23-s002.

Ahmed SF, Shabayek MI, Ghany Abdel ME, El-Hefnawy MH, El-Mesallamy HO. 2018. Role of CTRP3, CTRP9 and MCP-1 for the Evaluation of T2DM Associated Coronary Artery Disease in Egyptian Postmenopausal Females. PLoS ONE, 13(12): 1-13. https://doi.org/10.1371%2Fjournal.pone.0208038.

Akash MSH, Rehman K and Liaqat A. 2018. Tumor Necrosis Factor-Alpha: Role in Development of Insulin Resistance and Pathogenesis of Type 2 Diabetes Mellitus. Journal of Cellular Biochemistry, 119: 105-110. doi: 10.1002/jcb.26174.

Cranford TL, Enos RT, Velazquez KT, McClellan JL, Davis JM, Singh UP, et al. 2016. Role of MCP-1 on Inflammatory Processes and Metabolic Dysfunction Following High-Fat Feedings in the FVB/N Strain. International Journal of Obesity, 40(5): 844-51. https://doi.org/10.1038/ijo.2015.244.

Frühbeck G, Catalan V, Ramirez B, Valenti V, Becerril Sara, Rodriguez A, et al. 2022. Serum Levels of IL-1 RA Increase with Obesity and Type 2 Diabetes in Relation to Adipose Tissue Dysfunction and are Reduced After Bariatric Surgery in Parallel to Adiposity. Journal of Inflammation Research, 15: 1331-1345. https://doi.org/10.2147/JIR.S354095.

Garcia AG, Rodriguez MR, Alonso CG, Ochoa DYR, and Aguilar CA. 2015. Myeloperoxidase is Associated with Insulin Resistance and Inflammation in Overweight Subjects with First-Degree Relatives with Type 2 Diabetes Mellitus. Diabetes Metabolism Journal, 39(1): 59-65. https://doi.org/10.4093%2Fdmj.2015.39.1.59.

Gurgel Maria HC, Montenegro Junior RM, Melo Ponte CM, Sousa TCS, Silva PGB, de Saosa BL, et al. 2017. Metabolic Syndrome, Diabetes and Inadequate Lifestyle in First-Degree Relatives of Acute Myocardial Infarction Survivors Younger Than 45 Years Old. Lipids in Health and Disease, 16(224):1–7. https://doi.org/10.1186/s12944-017-0605-4.

Hu X, Pan X, Bu X, Y Luo, Xu Y, Xiong Q, et al. 2016. Contribution of a First-Degree Family History of Diabetes to Increased Serum Adipocyte Fatty Acid Binding Protein Levels Independent of Body Fat Content and Distribution. International Journal of Obesity, 40(11): 1649–1654. doi: 10.1038/ijo.2016.147.

Hu Xiang, Yu W, Yang L, Pan W, Si Q, Li Q, et al. 2019. The Association between First-Degree Family History of Diabetes and Metabolic Syndrome. Endocrine Practice, 25(7): 678–83. https://doi.org/10.4158/ep-2018-0543.

Jing X, Chen J, Dong Y, Han D, Zhao H, Wang X, Gao F, Li C, Cui Z, Liu Y, Ma J. 2018. Related factors of quality of life of type 2 diabetes patients: a systematic review and meta-analysis. Health Qual Life Outcomes. 16(1):189. https://doi.org/10.1186/s12955-018-1021-9.

Kamei N, Tobe K, Suzuki R, et al. 2006. Overexpression of Macrophage Chemoattractant Protein-1 in Adipose Tissue Cause Macrophage Recruitment and Insulin Resistance. The Journal of Biological Chemistry, 281: 26602-26614.http://dx.doi.org/10.1074/jbc.M601284200.

Kuroda M, Nishiguchi M, Ugawa N, Ishikawa E, Kawabata Y, et al. 2020. Interferon Regulatory Factor 7 Mediates Obesity-Associated MCP-1 Transcription. Plos one, 15(5): 1-21. https://doi.org/10.1371/journal.pone.0233390

Liu T, Guo Zixuan, Xueli L, Liu L, Dong Wenxiao, Wang S, et al. 2020. High-Fat Diet-Induced Dysbiosis Mediates MCP-1/CCR2 Axis-Dependent M2 Macrophage Polarization and Promotes Intestinal Adenoma-Adenocarcinoma Sequence. Journal of Cellular and Molecular Medicine, 24(4): 2648–2662. https://doi.org/10.1111/jcmm.14984

Michailidou Z, Gomez-Salazar M and Alexaki VI. 2022. Innate Immune Cells in the Adipose Tissue in Health and Metabolic Disease. Journal of Innate Immunity, 14(1): 4-30. doi: 10.1159/000515117.

Mittal M, Gupta H, and Deepshika. 2018. The Study of Prevalence of Metabolic Syndrome in the First Degree Relatives of Type 2 Diabetes Mellitus Patients. International Journal Research Medical Sciences, 6(9): 3042-3046. https://doi.org/10.18203/2320-6012.ijrms20183641.

Moon JH, Kwak SH, Jang HC. 2017. Prevention of type 2 diabetes mellitus in women with previous gestational diabetes mellitus. Korean J Intern Med. 32(1):26-41. doi: 10.3904/kjim.2016.203.

Panee Jun. 2012. Monocyte Chemoattractant Protein 1 (MCP-1) in Obesity and Diabetes. Cytokine, 60(1): 1–12. doi: 10.1016/j.cyto.2012.06.018.

Salvador AC, Arend Danny, Barrington WT, Elsaadi Ahmed M, Brockman GA, Threadgil David W, et al. 2021. Sex-Specific Genetic Architecture in Response to American and Ketogenic Diets. Internation Journal of Obesity, 45: 1284–1297 https://doi.org/10.1038/s41366-021-00785-7.

Syarif, Rasyid H, Aman M, Lawrence GS. 2021. High-Fat Diet Increases the Level of Circulating Monocyte Chemoattractant Protein-1 in Wistar Rats, Independent of Obesity. Annals of Medicine and Surgery, 65: 1-4. https://doi.org/10.1016%2Fj.amsu.2021.102266.