Giving of Diethylnitrisamine and Carbon Tetrachloride on Histological Appearance of Rat Kidney (Rattus Norvegicus)

Almas Dwi Khairana; Ricadonna  Raissa; Wibi Riawan; Suharti Suharti; Hendra Susanto; Aulanni’am Aulanni’am

doi:10.36590/jika.v4i1.242

Authors

Almas Dwi Khairana Universitas Negeri Malang
Ricadonna Raissa Universitas Brawijaya
Wibi Riawan Universitas Brawijaya
Suharti Universitas Negeri Malang
Hendra Susanto Universitas Negeri Malang
Aulanni’am Aulanni’am Universitas Brawijaya

DOI:

https://doi.org/10.36590/jika.v4i1.242

Keywords:

diethylnitrosamin, kidney, glomerulus

Abstract

Diethylnitrosamine (DEN) is a metabolic product of various foods and beverages in which there are preservatives in the form of nitrate and nitrite. Nitrosamines are found in various products such as alcoholic beverages, processed meats, cigarettes, and cosmetic products. DEN is toxic and carcinogenic to organs such as the liver, lungs, kidneys, skin, stomach, and blood. In addition to giving DEN compounds, there are also CCl4 compounds as tumor promoter agents known as genotoxic substances that can increase the risk of genetic errors and stimulate cells to become malignant neoplasms. Administration of DEN and CCl4 induction can damage various organs of the body, one of which is the kidney. In a healthy body, angiogenesis plays a role in wound healing and the formation of new tissue. However, angiogenesis also contributes to carcinogenesis or the uncontrolled growth of cancer cells, and is malignant when the body is contaminated with toxic substances. Therefore, to determine the effect of the administration of DEN and CCl4 observations were made using the immunohistochemical method, to see differences in the histology of the kidneys of experimental animals, the positive control group, and the negative control group based on the VEGF angiogenesis factor. From these results, there are significant differences. In the negative group, there was no necrosis and high-value VEGF expression, but in the positive group, there was necrosis and a decrease in the value of VEGF expression compared to the negative control group.

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Author Biographies

Almas Dwi Khairana, Universitas Negeri Malang

Prodi Kimia, Fakultas Matematika dan IPA, Universitas Negeri Malang, Malang, Indonesia

Ricadonna Raissa, Universitas Brawijaya

Prodi Kimia, Fakultas Matematika dan IPA, Universitas Brawijaya, Malang, Indonesia

Wibi Riawan, Universitas Brawijaya

Departemen Biokimia, Fakultas Kedokteran, Universitas Brawijaya, Malang, Indonesia

https://scholar.google.co.id

Suharti, Universitas Negeri Malang

Prodi Biologi, Fakultas Matematika dan IPA, Universitas Negeri Malang, Malang, Indonesia

https://scholar.google.co.id

Hendra Susanto, Universitas Negeri Malang

Departemen Biologi, Fakultas Matematika dan IPA, Universitas Negeri Malang, Malang, Indonesia

https://scholar.google.co.id

Aulanni’am Aulanni’am, Universitas Brawijaya

Prodi Biokimia, Fakultas Kedokteran Hewan, Universitas Brawijaya, Malang, Indonesia

https://scholar.google.com

References

Al-Kaseem M, Al-Assaf Z K, F. 2014. Determination of Seven Volatile N-Nitrosamines in Fast Food. Pharmacol Pharm.

Angmalisang EC. 2020. Peran Sinyal Ephrin-B2/EPH-B4 pada Angiogenesis Postnatal. Jurnal Biomedik: JBM, 12(2): 77. https://doi.org/10.35790/jbm.12.2.2020.29161.

Arief H, Widodo MA. 2018. Peranan Stres Oksidatif pada Proses Penyembuhan Luka. Jurnal Ilmiah Kedokteran Wijaya Kusuma, 5(2): 22. https://doi.org/10.30742/jikw.v5i2.338.

Falcon BL, Chintharlapalli S, Uhlik MT, Pytowsk B. 2016. Antagonist antibodies to vascular endothelial growth factor receptor 2 (VEGFR-2) as anti-angiogenic agents. Pharmacology & Therapeutics 164: 204–225.

Fitriani U, Dewi T friska, Wijayanti E. 2019. Analisis fungsi hati dan fungsi ginjal pada tikus setelah pemberian ramuan cabe Jawa, daun sendok dan seledri. Pros Sem Nas Masy Biodiv Indon, 5(2): 263–266. https://doi.org/10.13057/psnmbi/m050220.

Frizani NA. Miranti IP. 2018. Pengaruh Daun Sukun (Artocarpus Altilis) terhadap Gambaran Fibrosis Hepar Tikus Wistar Yang Diinduksi Dietilnitrosamin. Diponegoro Medical Journal (Jurnal Kedokteran Diponegoro), 7(2): 1072-1080.

Hatcher JM, Wu G, Zeng C, Zhu J, Meng F, Patel S, Wang W, Ficarro SB, Leggett AL, Powell CE, Marto JA, Zhang K, Ngo JCK, Fu X-D, Zhang T, Gray S, N. 2018. SRPKIN-1: A Covalent SRPK1/2 Inhibitor that Potently Converts VEGF from Pro-angiogenic to Anti-angiogenic Isoform. Cell Chemical Biology. Cell Chemical Biology, 25(4):460-470.e6.

Istikhomah, Lisdiana. 2015. Efek Hepatoprotektor Ekstrak Buah Pedada (Sonneratia caseolaris) Pada Tikus Putih (Rattus norvegicus. Unnes Journal of Life Science, 4(1): 1–8.

Hasanatuludhhiyah, Basori A, Suhartati. 2016. Gangguan Ginjal Akut Akibat Rhabdomiolisis. Majalah Biomorfologi, 28: 1–23.

Panjaitan RGP, Handharyani E, Chairul C, Masriani M, Zakiah Z, Manalu W. 2010. The Effects of Carbon Tetrachloride Administration on Liver and Renal Function. Makara Journal of Health Research, 11(1): 11–16. https://doi.org/10.7454/msk.v11i1.217.

Purnamasari P, Purnawati RD. Susilaningsih N. 2018. Pengaruh Ekstrak Daun Sukun dan Madu terhadap Gambaran Mikroskopik Ginjal Tikus Wistar Yang Diinduksi Dietilnitrosamin. Diponegoro Medical Journal (Jurnal Kedokteran Diponegoro), 7(2) 1391-1405.

Robles H. 2014. Encyclopedia of Toxicology, Nitrosamine. Academic Press.

Rohamniah AU. 2021. Efek Renoprotektif Ekstrak Ganoderma Applanatum Terhadap Kadar Asam Hialuronat dan Laminin Fibrosis Ginjal Mencit (Mus musculus L.) yang Terinduksi Karbontetraklorida. Universitas Airlangga: Surabaya.

Rosen LS. 2002. Clinical Experience with Angiogenesis Signaling Inhibitors: Focus on Vascular Endothelial Growth Factor (VEGF) Blockers. Supplemen, 9(2): 36-44.

Rosen S, Stillman IE. 2008. Acute tubular necrosis is a syndrome of physiologic and pathologic dissociation. Journal of the American Society of Nephrology, 19(5): 871–875. https://doi.org/10.1681/ASN.2007080913.

Santos NP, Colaço AA, Oliveira PA. 2017. Animal models as a tool in hepatocellular carcinoma research. https://doi.org/10.1177/1010428317695923.

Singh D, Chaudhary D, Kumar V, Verma A. 2021. Amelioration of diethylnitrosamine (DEN) induced renal oxidative stress and inflammation by Carissa carandas embedded silver nanoparticles in rodents. Toxicology Reports. https://doi.org/10.1016/j.toxrep.2021.03.014.

Soichiro Sasaki A, Tomohisa B, Tatsunori N, Yoshihiro H, Shin-ichi H, Noriko Gotoh NM. 2016. Essential roles of the interaction between cancer cell-derived chemokine, CCL4, and intra-bone CCR5-expressing ?broblasts in breast cancer bone metastasis. Cancer Letters, 378(1): 23-32.

Suzek H, Celik I, Dogan A YS. 2016. Protective effect and antioxidant role of sweetgum (Liquidambar orientalis) oil against carbon tetrachloride-induced hepatotoxicity and oxidative stress in rats. Pharmaceutical Biology, 54(3): 451-7.

Tang D, Hu S, Dai F, Yi R, Gordin ML CS. 2016. Self-Templated Synthesis of Mesoporous Carbon from Carbon Tetrachloride Precursor for Supercapacitor Electrodes. ACS Omega, 4(15): 16360–16371.

Wahyuningrum MR, Probosari. 2012. Pengaruh Pemberian Buah Pepaya (Carica Papaya L.) Terhadap Kadar Trigliserida Pada Tikus Sprague Dawley Dengan Hiperkolesterolemia. Journal of Nutrition College, 1(1): 192-198. https://doi.org/10.14710/jnc.v1i1.693.

Wicaksono S. 2019. Pengaruh Pemberian Spirulina Peroral Yang Diberi CCl4 terhadap Kadar Ureum dan Kreatinin Darah Tikus Putih. Jurnal Kesehatan, 12(2): 86-91. https://doi.org/10.24252/kesehatan.v12i2.9829.

Yulai Y, Fengfeng Z, Zhenhuan L, LingFeng Z, Yu X, Arunachalam C, Tahani A, Alamahdi BL. 2021. Phyllanthin prevents diethylnitrosamine (DEN) induced liver carcinogenesis in rats and induces apoptotic cell death in HepG2 cells. Biomedicine & Pharmacotherapy, 135: 111335. https://doi.org/10.1016/j.biopha.2021.111335.